Hotspot

trnN-GUU-ndhF

IRb · 112016-113495 · 1480 bp

Back to Disanthus

Pi 0

MarkerSeek score 59.80

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1480
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	ACGACCAGTCAGTTAACAGC	GGTGGGCGCATTTCTTCTTA	50.0	50.0	57.8	58.5	1597-1613 bp	1.0	75.3
trnN-GUU-ndhF_p2	AGGATTCGAACCTACGACCA	GGTGGGCGCATTTCTTCTTA	50.0	50.0	58.2	58.5	1610-1626 bp	1.0	75.2
trnN-GUU-ndhF_p3	GGATTCGAACCTACGACCAG	GGTGGGCGCATTTCTTCTTA	55.0	50.0	57.8	58.5	1609-1625 bp	1.0	74.8
trnN-GUU-ndhF_p4	CGAGCCCTTTCATCCTGAAT	GGTGGGCGCATTTCTTCTTA	50.0	50.0	57.7	58.5	1664-1680 bp	1.0	73.7
trnN-GUU-ndhF_p5	TTCGAACCTACGACCAGTCA	GGTGGGCGCATTTCTTCTTA	50.0	50.0	58.4	58.5	1606-1622 bp	1.0	73.3