Hotspot

rps16-trnQ-UUG

LSC · 6270-7703 · 1434 bp

Back to Disanthus

Pi 0

MarkerSeek score 60.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1434
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	TAGAAACTCGAAATGGGGCG	45.0	50.0	58.1	58.0	1547-1568 bp	1.0	79.8
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1486-1507 bp	1.0	79.4
rps16-trnQ-UUG_p3	TCATGTCCTTCAAGTCGCAC	TAGAAACTCGAAATGGGGCG	50.0	50.0	58.2	58.0	1558-1579 bp	1.0	79.3
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1497-1518 bp	1.0	78.9
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	AAACTCGAAATGGGGCGTAG	45.0	50.0	58.1	58.3	1544-1565 bp	1.0	77.8