Hotspot

psbE-petL

LSC · 66927-67716 · 790 bp

Back to Dipteronia

Pi 0.0497

MarkerSeek score 83.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 790
variable_sites: 38
pi: 0.0497
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TCACAAATAACCAACCCGCA	AGCCGAGGTTAGAGTTAAAGC	45.0	47.6	58.0	57.8	965-971 bp	1.0	75.2
psbE-petL_p2	TCACAAATAACCAACCCGCA	GAGGTTAGAGTTAAAGCCGCT	45.0	47.6	58.0	57.8	961-967 bp	1.0	75.2
psbE-petL_p3	CAACCCGCAATGAATAGGGA	AGCCGAGGTTAGAGTTAAAGC	50.0	47.6	57.9	57.8	954-960 bp	1.0	75.0
psbE-petL_p4	CAACCCGCAATGAATAGGGA	GAGGTTAGAGTTAAAGCCGCT	50.0	47.6	57.9	57.8	950-956 bp	1.0	75.0
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	GAGGTTAGAGTTAAAGCCGCT	50.0	47.6	57.8	57.8	873-879 bp	1.0	74.6