Hotspot

rps4-trnT-UGU

LSC · 47809-48175 · 367 bp

Back to Dipteronia

Pi 0.103

MarkerSeek score 76.30

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 367
variable_sites: 34
pi: 0.103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CTCGTTAGTCCCGGTAAAGC	TTTAAGCCCGCTTAGCTCAG	55.0	50.0	58.1	58.0	506-513 bp	1.0	88.7
rps4-trnT-UGU_p2	CTCGTTAGTCCCGGTAAAGC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.1	57.9	491-498 bp	1.0	87.9
rps4-trnT-UGU_p3	CTCGTTAGTCCCGGTAAAGC	ATGGTCATCGGTTCGATTCC	55.0	50.0	58.1	57.8	459-466 bp	1.0	87.5
rps4-trnT-UGU_p4	CTCGTTAGTCCCGGTAAAGC	CCGCTTAGCTCAGAGGTTAG	55.0	55.0	58.1	57.5	499-506 bp	1.0	84.9
rps4-trnT-UGU_p5	CTCGTTAGTCCCGGTAAAGC	CAGAGGTTAGAGCATCGCAT	55.0	50.0	58.1	57.5	489-496 bp	1.0	84.9