Hotspot

psbZ-trnG-UCC

LSC · 37334-37895 · 562 bp

Back to Dipteronia

Pi 0.0351

MarkerSeek score 73.80

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 562
variable_sites: 19
pi: 0.0351
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	AGTCTTTCTGGTGGGTATCCT	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	57.8	57.8	641-649 bp	1.0	73.8
psbZ-trnG-UCC_p2	AGTCTTTCTGGTGGGTATCC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.2	57.8	641-649 bp	1.0	69.3
psbZ-trnG-UCC_p3	GTCTTTCTGGTGGGTATCCT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.2	57.8	640-648 bp	1.0	69.3
psbZ-trnG-UCC_p4	GTCTTTCTGGTGGGTATCCTT	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	57.0	57.8	640-648 bp	1.0	67.5
psbZ-trnG-UCC_p5	TGGATTAGTCTTTCTGGTGGG	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	57.0	57.8	647-655 bp	1.0	67.4