Hotspot

trnE-UUC-trnT-GGU

LSC · 31704-32494 · 791 bp

Back to Dipteronia

Pi 0.0421

MarkerSeek score 72.70

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 791
variable_sites: 32
pi: 0.0421
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AAAGAAGAAAGCAGCACGGA	GAACCGATGACTTACGCCTT	45.0	50.0	58.0	58.0	1368-1390 bp	1.0	83.7
trnE-UUC-trnT-GGU_p2	AAAGAAGAAAGCAGCACGGA	CCATGGCGTTACTCTACCAC	45.0	55.0	58.0	58.1	1347-1369 bp	1.0	83.3
trnE-UUC-trnT-GGU_p3	AAAGAAGAAAGCAGCACGGA	GATCTTCGTGTGTTCGGGAA	45.0	50.0	58.0	57.9	1991-2002 bp	1.0	83.2
trnE-UUC-trnT-GGU_p4	AAAGAAGAAAGCAGCACGGA	TCTTCGTGTGTTCGGGAATC	45.0	50.0	58.0	57.9	1989-2000 bp	1.0	83.2
trnE-UUC-trnT-GGU_p5	AAAGAAGAAAGCAGCACGGA	TGGCGTTACTCTACCACTGA	45.0	50.0	58.0	58.1	1344-1366 bp	1.0	83.1