Hotspot

petN-psbM

LSC · 29508-30277 · 770 bp

Back to Dipteronia

Pi 0.0297

MarkerSeek score 73.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 770
variable_sites: 22
pi: 0.0297
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TATGGGGAAGGAGTGGACTC	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.8	57.6	857-872 bp	1.0	72.1
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	917-932 bp	1.0	72.0
petN-psbM_p3	GGGGAAGGAGTGGACTCTAG	TGCTACTGCACTGTTCATTCT	60.0	42.9	58.2	57.6	854-869 bp	1.0	71.8
petN-psbM_p4	CTTGGGCTGCGTTAATGGTA	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.3	57.6	905-920 bp	1.0	71.3
petN-psbM_p5	TTGGGCTGCGTTAATGGTAG	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.3	57.6	904-919 bp	1.0	71.3