Hotspot

atpI-rps2

LSC · 16165-16384 · 220 bp

Back to Dipteronia

Pi 0.15

MarkerSeek score 75.30

Primer available yes

Label atpI-rps2

Feature Metadata

label_name: atpI-rps2
feature_type: igs
region: LSC
length_bp: 220
variable_sites: 33
pi: 0.15
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-rps2_p1	CTTTTAGTGTGTTACTGGAACA	TTGTGAGGGTCGTTCTAGCT	36.4	50.0	54.3	58.4	294-302 bp	1.0	48.0
atpI-rps2_p2	CTTTTAGTGTGTTACTGGAACA	TTTGTGAGGGTCGTTCTAGC	36.4	50.0	54.3	57.5	295-303 bp	1.0	48.0
atpI-rps2_p3	CTTTTAGTGTGTTACTGGAACA	TGTGAGGGTCGTTCTAGCTA	36.4	50.0	54.3	57.5	293-301 bp	1.0	48.0
atpI-rps2_p4	TTTTAGTGTGTTACTGGAACA	TTGTGAGGGTCGTTCTAGCT	33.3	50.0	52.9	58.4	293-301 bp	1.0	48.0
atpI-rps2_p5	TTTTAGTGTGTTACTGGAACA	TTTGTGAGGGTCGTTCTAGC	33.3	50.0	52.9	57.5	294-302 bp	1.0	48.0