Hotspot

trnG-trnR-UCU

LSC · 10558-10727 · 170 bp

Back to Dipteronia

Pi 0.0859

MarkerSeek score 81.90

Primer available yes

Label trnG-trnR-UCU

Feature Metadata

label_name: trnG-trnR-UCU
feature_type: igs
region: LSC
length_bp: 170
variable_sites: 14
pi: 0.0859
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	250-253 bp	1.0	70.0
trnG-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	253-256 bp	1.0	69.7
trnG-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	256-259 bp	1.0	69.2
trnG-trnR-UCU_p4	AGCCTTCCAAGCTAACGATG	TGCTAAATTGCGTCCAATAGGA	50.0	40.9	58.0	58.1	294-297 bp	1.0	68.5
trnG-trnR-UCU_p5	CCTAGCCTTCCAAGCTAACG	TGCTAAATTGCGTCCAATAGGA	55.0	40.9	58.1	58.1	297-300 bp	1.0	68.2