Hotspot

rpl32-ndhF

SSC · 118936-119882 · 947 bp

Back to Dipterocarpus

Pi 0.0109

MarkerSeek score 61.60

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 947
variable_sites: 30
pi: 0.0109
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CAAAGCTTTCAACGCTGTCC	TTGGAAGTGGACTGAAAGGT	50.0	45.0	58.2	56.6	1093-1118 bp	1.0	69.4
rpl32-ndhF_p2	CCCTTCTTCTTCCACACGTT	TTGGAAGTGGACTGAAAGGT	50.0	45.0	57.7	56.6	1068-1093 bp	1.0	69.3
rpl32-ndhF_p3	TCAACGCTGTCCAATATCCC	TTGGAAGTGGACTGAAAGGT	50.0	45.0	57.7	56.6	1085-1110 bp	1.0	68.8
rpl32-ndhF_p4	CAACGCTGTCCAATATCCCT	TTGGAAGTGGACTGAAAGGT	50.0	45.0	57.7	56.6	1084-1109 bp	1.0	68.7
rpl32-ndhF_p5	AGCTTTCAACGCTGTCCAAT	TTGGAAGTGGACTGAAAGGT	45.0	45.0	58.4	56.6	1090-1115 bp	1.0	68.2