Hotspot

trnN-GUU-ycf1

IRb · 101541-102035 · 495 bp

Back to Dipterocarpus

Pi 0.0202

MarkerSeek score 61.40

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: IRb
length_bp: 495
variable_sites: 26
pi: 0.0202
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	ATAGCGCAGAACAGAATGGG	GAGCTCGGAGAAGGAAGAGA	50.0	55.0	58.0	58.2	688-949 bp	1.0	84.4
trnN-GUU-ycf1_p2	TCGGGAGAATCGATGACTGA	GAGCTCGGAGAAGGAAGAGA	50.0	55.0	57.9	58.2	834-1095 bp	1.0	83.9
trnN-GUU-ycf1_p3	AGCTATGTAACTTGGGCGTG	GAGCTCGGAGAAGGAAGAGA	50.0	55.0	58.0	58.2	1095-1346 bp	1.0	83.8
trnN-GUU-ycf1_p4	TTTTGGTTTTACGCCCCGTA	GAGCTCGGAGAAGGAAGAGA	45.0	55.0	58.0	58.2	972-1232 bp	1.0	83.8
trnN-GUU-ycf1_p5	GGCAGAATAGCAGAGCAAGT	GAGCTCGGAGAAGGAAGAGA	50.0	55.0	58.0	58.2	931-1191 bp	1.0	83.8