Hotspot

rps4-trnT-UGU

LSC · 39629-40213 · 585 bp

Back to Dipterocarpus

Pi 0.0178

MarkerSeek score 66.10

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 585
variable_sites: 29
pi: 0.0178
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	AGTTAGTCCCGGTAAAGCCC	AGTATTTGAGCCCGCTTAGC	55.0	50.0	59.1	58.0	692-744 bp	1.0	78.1
rps4-trnT-UGU_p2	AGTTAGTCCCGGTAAAGCCC	GTATTTGAGCCCGCTTAGCT	55.0	50.0	59.1	58.0	691-743 bp	1.0	78.1
rps4-trnT-UGU_p3	CCCCAGACGGCGTATTTTT	AGTATTTGAGCCCGCTTAGC	52.6	50.0	58.1	58.0	674-726 bp	1.0	77.8
rps4-trnT-UGU_p4	CCCCAGACGGCGTATTTTT	GTATTTGAGCCCGCTTAGCT	52.6	50.0	58.1	58.0	673-725 bp	1.0	77.8
rps4-trnT-UGU_p5	AGTTAGTCCCGGTAAAGCCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	59.1	57.9	673-725 bp	1.0	77.5