Hotspot

psbZ-trnG-UCC

LSC · 28955-29685 · 731 bp

Back to Dipterocarpus

Pi 0.0067

MarkerSeek score 63.40

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 731
variable_sites: 13
pi: 0.0067
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	CTTCTCCTGATGGTTGGGTG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	57.9	57.8	856-889 bp	1.0	82.9
psbZ-trnG-UCC_p2	CCCGTTGTTTTTGCTTCTCC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.9	57.8	869-902 bp	1.0	82.7
psbZ-trnG-UCC_p3	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	859-892 bp	1.0	82.2
psbZ-trnG-UCC_p4	TTCTCCTGATGGTTGGGTGA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.3	57.8	855-888 bp	1.0	81.8
psbZ-trnG-UCC_p5	CTTCTCCTGATGGTTGGGTG	TAAATACATTGCCTGGGCGG	55.0	50.0	57.9	58.3	895-928 bp	1.0	81.7