Hotspot

trnE-UUC-psbD

LSC · 23125-25655 · 2531 bp

Back to Dipterocarpus

Pi 0.0043

MarkerSeek score 58.50

Primer available yes

Label trnE-UUC-psbD

Feature Metadata

label_name: trnE-UUC-psbD
feature_type: igs
region: LSC
length_bp: 2531
variable_sites: 24
pi: 0.0043
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-psbD_p1	CGTAGTATCCTACCCCCAGG	GAAACGGTCCCTACGTAACC	60.0	55.0	58.1	58.0	2593-2698 bp	1.0	84.0
trnE-UUC-psbD_p2	GCTGCCTCCTTGAAAGAGAG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.3	58.0	2558-2663 bp	1.0	82.6
trnE-UUC-psbD_p3	TGTCCTGAACCACTAGACGA	GAAACGGTCCCTACGTAACC	50.0	55.0	57.7	58.0	2537-2642 bp	1.0	82.5
trnE-UUC-psbD_p4	CGTAGTATCCTACCCCCAGG	ACAAAAACGAAACGGTCCCT	60.0	45.0	58.1	58.2	2601-2706 bp	1.0	82.0
trnE-UUC-psbD_p5	CGTAGTATCCTACCCCCAGG	GACCGGACCAACCTACAAAA	60.0	50.0	58.1	57.7	2615-2720 bp	1.0	81.9