Hotspot

ycf1

IRb · 113351-115306 · 1956 bp

Back to Dipsacus

Pi 0.0048

MarkerSeek score 50.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1956
variable_sites: 14
pi: 0.0048
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGCCATCCATCCATATCCCA	TAGGAACTGGGAGTGGAAGG	50.0	55.0	57.9	58.0	2652-2709 bp	1.0	81.9
ycf1_p2	ACGCGGTTAGGATCGATCTA	TAGGAACTGGGAGTGGAAGG	50.0	55.0	58.1	58.0	2433-2490 bp	1.0	81.7
ycf1_p3	TTTCACGTCGTGTACAGGAC	TAGGAACTGGGAGTGGAAGG	50.0	55.0	57.9	58.0	2597-2654 bp	1.0	81.5
ycf1_p4	AGCCATCCATCCATATCCCA	GGGAGTGGAAGGAAGGGTAT	50.0	55.0	57.9	58.1	2644-2701 bp	1.0	81.4
ycf1_p5	ACGCGGTTAGGATCGATCTA	AACCAAAATCCCCAACACGA	50.0	45.0	58.1	57.9	2940-2997 bp	1.0	81.1