Hotspot

ccsA

SSC · 118948-119913 · 966 bp

Back to Dipsacus

Pi 0.00414

MarkerSeek score 40.50

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 966
variable_sites: 6
pi: 0.00414
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GGCAGTCCGTCTTCTAAAAGA	GGTTCTCGCCAATTCGTACA	47.6	50.0	57.7	58.3	1136 bp	1.0	67.6
ccsA_p2	GGCAGTCCGTCTTCTAAAAG	GGTTCTCGCCAATTCGTACA	50.0	50.0	56.2	58.3	1136 bp	1.0	63.5
ccsA_p3	GGCAGTCCGTCTTCTAAAAGA	GGGTTCTCGCCAATTCGTAC	47.6	55.0	57.7	59.0	1137 bp	1.0	61.9
ccsA_p4	GGCAGTCCGTCTTCTAAAAGA	TCTCGCCAATTCGTACAAAGT	47.6	42.9	57.7	58.0	1133 bp	1.0	61.6
ccsA_p5	GGCAGTCCGTCTTCTAAAAGA	CACAGGGTTCTCGCCAATT	47.6	52.6	57.7	58.1	1141 bp	1.0	61.5