Hotspot

rps15-ycf1

SSC · 128040-128438 · 399 bp

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Pi 0.00334

MarkerSeek score 53.20

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 399
variable_sites: 2
pi: 0.00334
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCAGCAGATCCCCTGTTTTC	GGCAGGAATCGTTTGAGGAT	50.0	50.0	57.5	58.0	553 bp	1.0	76.0
rps15-ycf1_p2	CAGCAGATCCCCTGTTTTCT	GGCAGGAATCGTTTGAGGAT	50.0	50.0	57.5	58.0	552 bp	1.0	75.9
rps15-ycf1_p3	TCAGCAGATCCCCTGTTTTC	CACCATTAGAACGGCAGGAA	50.0	50.0	57.5	57.9	565 bp	1.0	75.4
rps15-ycf1_p4	CAGCAGATCCCCTGTTTTCT	CACCATTAGAACGGCAGGAA	50.0	50.0	57.5	57.9	564 bp	1.0	75.4
rps15-ycf1_p5	TCAGCAGATCCCCTGTTTTC	ATCCACCATTAGAACGGCAG	50.0	50.0	57.5	57.7	568 bp	1.0	73.6