Hotspot

ccsA-ndhD

SSC · 119914-120162 · 249 bp

Back to Dipsacus

Pi 0.0215

MarkerSeek score 52.10

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 249
variable_sites: 8
pi: 0.0215
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCTCTTCAATCCGAGTGGTT	TGGACCGCGAGAGTTATTTG	50.0	50.0	57.9	58.0	971-973 bp	1.0	80.2
ccsA-ndhD_p2	GCTCTTCAATCCGAGTGGTT	GGACCGCGAGAGTTATTTGT	50.0	50.0	57.9	58.0	970-972 bp	1.0	80.2
ccsA-ndhD_p3	GCTCTTCAATCCGAGTGGTT	CGTTTTTGATTCTGGACCGC	50.0	50.0	57.9	58.0	983-985 bp	1.0	80.0
ccsA-ndhD_p4	GCTCTTCAATCCGAGTGGTT	GTTTTTGATTCTGGACCGCG	50.0	50.0	57.9	58.0	982-984 bp	1.0	80.0
ccsA-ndhD_p5	GCTCTTCAATCCGAGTGGTT	TACAGGACTCAATGGAGCCA	50.0	50.0	57.9	58.0	1367-1369 bp	1.0	79.6