Hotspot

trnG-GCC-trnfM-CAU

LSC · 37948-38131 · 184 bp

Back to Dipsacus

Pi 0.0109

MarkerSeek score 60.00

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 184
variable_sites: 3
pi: 0.0109
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	ATGCGGATATGGTCGAATGG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.9	58.0	289-291 bp	1.0	80.0
trnG-GCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	260-262 bp	1.0	79.6
trnG-GCC-trnfM-CAU_p3	ATGCGGATATGGTCGAATGG	ATTAGTTTTCAGCGCGGAGT	50.0	45.0	57.9	57.9	341-343 bp	1.0	79.4
trnG-GCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	ATTAGTTTTCAGCGCGGAGT	50.0	45.0	57.8	57.9	312-314 bp	1.0	78.9
trnG-GCC-trnfM-CAU_p5	ATGCGGATATGGTCGAATGG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.9	57.8	287-289 bp	1.0	78.9