Hotspot

psbM-trnD-GUC

LSC · 31507-32441 · 935 bp

Back to Dipsacus

Pi 0.00927

MarkerSeek score 64.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 935
variable_sites: 13
pi: 0.00927
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAAAGGCGGTAGGGACTAG	GCTAGATCCCCTCTTTCCCT	55.0	55.0	58.2	57.9	1094-1106 bp	1.0	78.3
psbM-trnD-GUC_p2	GAAAGGCGGTAGGGACTAGA	GCTAGATCCCCTCTTTCCCT	55.0	55.0	58.2	57.9	1093-1105 bp	1.0	78.2
psbM-trnD-GUC_p3	AAAGGCGGTAGGGACTAGAA	GCTAGATCCCCTCTTTCCCT	50.0	55.0	57.8	57.9	1092-1104 bp	1.0	78.1
psbM-trnD-GUC_p4	AAGTAGAAAGGCGGTAGGGA	GCTAGATCCCCTCTTTCCCT	50.0	55.0	57.8	57.9	1098-1110 bp	1.0	78.1
psbM-trnD-GUC_p5	AGGCGGTAGGGACTAGAATG	GCTAGATCCCCTCTTTCCCT	55.0	55.0	58.3	57.9	1090-1102 bp	1.0	77.7