Hotspot

rps2-rpoC2

LSC · 17525-17710 · 186 bp

Back to Dipsacus

Pi 0.0179

MarkerSeek score 63.90

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 186
variable_sites: 5
pi: 0.0179
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CCATGGCCAAAATGAACTCC	GATACGCCGGAACAATCAGT	50.0	50.0	57.3	58.1	332 bp	1.0	75.3
rps2-rpoC2_p2	CCATGGCCAAAATGAACTCC	TACGATACGCCGGAACAATC	50.0	50.0	57.3	57.9	335 bp	1.0	74.8
rps2-rpoC2_p3	GCCAAAATGAACTCCTGCCT	GATACGCCGGAACAATCAGT	50.0	50.0	58.7	58.1	327 bp	1.0	74.8
rps2-rpoC2_p4	CCATGGCCAAAATGAACTCC	CTACGATACGCCGGAACAAT	50.0	50.0	57.3	57.9	336 bp	1.0	74.7
rps2-rpoC2_p5	GCCAAAATGAACTCCTGCCT	TACGATACGCCGGAACAATC	50.0	50.0	58.7	57.9	330 bp	1.0	74.3