Hotspot

atpH-atpI

LSC · 14678-15860 · 1183 bp

Back to Dipsacus

Pi 0.00113

MarkerSeek score 59.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1183
variable_sites: 2
pi: 0.00113
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GCCAACTCCAGGTCCAATAG	TTTTTGCAACTTTAGCCGCG	55.0	45.0	58.0	58.5	1320 bp	1.0	75.7
atpH-atpI_p2	GCCAACTCCAGGTCCAATAG	CAAGCGGGATTCAAGCTCTT	55.0	50.0	58.0	58.5	1341 bp	1.0	75.5
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	57.9	58.5	1287 bp	1.0	74.9
atpH-atpI_p4	CAGCAGCAATAACAGAAGCG	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.8	58.5	1279 bp	1.0	74.7
atpH-atpI_p5	AGCCAATCCAGCAGCAATAA	CAAGCGGGATTCAAGCTCTT	45.0	50.0	57.9	58.5	1308 bp	1.0	74.7