Hotspot

trnG-GCC

LSC · 8690-8716 · 27 bp

Back to Dipodium

Pi 0.037

MarkerSeek score 65.60

Primer available yes

Label trnG-GCC

Feature Metadata

label_name: trnG-GCC
feature_type: tRNA
region: LSC
length_bp: 27
variable_sites: 1
pi: 0.037
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC_p1	TGAAAGTTGAAGTTCAGTATTCT	TGTCCTATTGAGGGGTAAAGGA	30.4	45.5	53.9	57.9	192 bp	1.0	40.4
trnG-GCC_p2	TCTAAATGAAAGTTGAAGTTCAGT	TGTCCTATTGAGGGGTAAAGGA	29.2	45.5	54.7	57.9	198 bp	1.0	40.4
trnG-GCC_p3	ATGAAAGTTGAAGTTCAGTATTCT	TGTCCTATTGAGGGGTAAAGGA	29.2	45.5	54.5	57.9	193 bp	1.0	40.4
trnG-GCC_p4	TGAAAGTTGAAGTTCAGTATTCT	TGTCCTATTGAGGGGTAAAGG	30.4	47.6	53.9	56.4	192 bp	1.0	40.4
trnG-GCC_p5	TGAAAGTTGAAGTTCAGTATTC	TGTCCTATTGAGGGGTAAAGGA	31.8	45.5	52.4	57.9	192 bp	1.0	40.4