Hotspot

ndhD

SSC · 109539-110717 · 1179 bp

Back to Dipodium

Pi 0.0738

MarkerSeek score 80.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1179
variable_sites: 71
pi: 0.0738
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AGTCAAGACTTTCTTTCCAA	CACGGGGTTTTCTGGTCTAA	35.0	50.0	52.2	57.4	1158-1284 bp	1.0	48.7
ndhD_p2	AGTCAAGACTTTCTTTCCAAT	CACGGGGTTTTCTGGTCTAA	33.3	50.0	52.9	57.4	1158-1284 bp	1.0	48.7
ndhD_p3	AGTCAAGACTTTCTTTCCAATT	CACGGGGTTTTCTGGTCTAA	31.8	50.0	53.8	57.4	1158-1284 bp	1.0	48.7
ndhD_p4	AGTCAAGACTTTCTTTCCAA	TTATTCTTCTCCCGAGCACG	35.0	50.0	52.2	57.4	1174-1300 bp	1.0	48.6
ndhD_p5	AGTCAAGACTTTCTTTCCAAT	TTATTCTTCTCCCGAGCACG	33.3	50.0	52.9	57.4	1174-1300 bp	1.0	48.6