Hotspot

clpP

LSC · 67530-69752 · 2223 bp

Back to Dipodium

Pi 0.0135

MarkerSeek score 51.90

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2223
variable_sites: 30
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ACTGAACGAGTCGCACATAC	CGAACAATCGGCTGATCTGA	50.0	50.0	58.0	58.1	2805-2810 bp	1.0	80.6
clpP_p2	CTGAACGAGTCGCACATACA	CGAACAATCGGCTGATCTGA	50.0	50.0	58.0	58.1	2804-2809 bp	1.0	80.5
clpP_p3	GTTGGCATGGCGTGTCTATA	CGAACAATCGGCTGATCTGA	50.0	50.0	58.1	58.1	2683-2688 bp	1.0	80.4
clpP_p4	ACTGAACGAGTCGCACATAC	ACAATCGGCTGATCTGATCG	50.0	50.0	58.0	57.9	2802-2807 bp	1.0	80.0
clpP_p5	CTGAACGAGTCGCACATACA	ACAATCGGCTGATCTGATCG	50.0	50.0	58.0	57.9	2801-2806 bp	1.0	80.0