Hotspot

rps3-rpl22

LSC · 81798-81843 · 46 bp

Back to Dipodium

Pi 0.0444

MarkerSeek score 80.00

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 46
variable_sites: 2
pi: 0.0444
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	TGTTCCAAGCCTGAAACCAA	AAAAGTTAAAACCCCGGGCT	45.0	45.0	57.8	57.9	182-183 bp	1.0	79.4
rps3-rpl22_p2	TTGTTCCAAGCCTGAAACCA	AAAAGTTAAAACCCCGGGCT	45.0	45.0	57.8	57.9	183-184 bp	1.0	79.4
rps3-rpl22_p3	CAAGCCTGAAACCAAGTGGA	AAAAGTTAAAACCCCGGGCT	50.0	45.0	58.3	57.9	177-178 bp	1.0	78.2
rps3-rpl22_p4	TCCAAGCCTGAAACCAAGTG	AAAAGTTAAAACCCCGGGCT	50.0	45.0	58.3	57.9	179-180 bp	1.0	78.1
rps3-rpl22_p5	TGTTCCAAGCCTGAAACCAA	AAAGTTAAAACCCCGGGCTC	45.0	50.0	57.8	58.4	181-182 bp	1.0	77.1