Hotspot

petA-psbJ

LSC · 60549-61604 · 1056 bp

Back to Dipodium

Pi 0.0201

MarkerSeek score 60.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1056
variable_sites: 20
pi: 0.0201
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGCATCAGAAGGACGTCAAG	TCCCTATTCATTGCGGGTTG	50.0	50.0	57.9	57.9	1982-2024 bp	1.0	82.7
petA-psbJ_p2	GCATCAGAAGGACGTCAAGT	TCCCTATTCATTGCGGGTTG	50.0	50.0	57.9	57.9	1981-2023 bp	1.0	82.7
petA-psbJ_p3	TGCATCAGAAGGACGTCAAG	CGGGTTGGTTATTCGTCAGT	50.0	50.0	57.9	57.9	1969-2011 bp	1.0	82.4
petA-psbJ_p4	GCATCAGAAGGACGTCAAGT	CGGGTTGGTTATTCGTCAGT	50.0	50.0	57.9	57.9	1968-2010 bp	1.0	82.4
petA-psbJ_p5	CCCCAATCGTATTTCTCCCG	TCCCTATTCATTGCGGGTTG	55.0	50.0	58.1	57.9	2315-2357 bp	1.0	81.9