Hotspot

atpB-rbcL

LSC · 51364-52314 · 951 bp

Back to Dipodium

Pi 0.0132

MarkerSeek score 70.70

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 951
variable_sites: 12
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGGGCCAATAATTTGAGCGA	TTTGTGGTGACATAAGGCCC	45.0	50.0	57.9	58.1	1045-1072 bp	1.0	82.4
atpB-rbcL_p2	CGATACGCCCTAGGTTTTGT	TTTGTGGTGACATAAGGCCC	50.0	50.0	57.7	58.1	1028-1055 bp	1.0	81.1
atpB-rbcL_p3	ACCCGGTGGAAAAATGACAT	TTTGTGGTGACATAAGGCCC	45.0	50.0	57.7	58.1	1072-1099 bp	1.0	81.0
atpB-rbcL_p4	CTGGGCCAATAATTTGAGCG	TTTGTGGTGACATAAGGCCC	50.0	50.0	57.5	58.1	1046-1073 bp	1.0	79.5
atpB-rbcL_p5	ACGCCCTAGGTTTTGTTCTT	TTTGTGGTGACATAAGGCCC	45.0	50.0	57.3	58.1	1024-1051 bp	1.0	78.4