Hotspot

psaA-ycf3

LSC · 40673-41317 · 645 bp

Back to Dipodium

Pi 0.00969

MarkerSeek score 68.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 645
variable_sites: 6
pi: 0.00969
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	797-822 bp	1.0	82.1
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	ATAGCGCTTACTCCTGGGAA	50.0	50.0	57.9	58.2	768-793 bp	1.0	80.8
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	TAGCGCTTACTCCTGGGAAT	50.0	50.0	57.9	58.2	767-792 bp	1.0	80.8
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	793-818 bp	1.0	80.3
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	CAAGCCGCTGAGTATTGGAA	50.0	50.0	57.9	58.3	795-820 bp	1.0	80.3