Hotspot

trnT-GGU-psbD

LSC · 31011-31915 · 905 bp

Back to Dipodium

Pi 0.0145

MarkerSeek score 53.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 905
variable_sites: 13
pi: 0.0145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGTTCAGGTTACGAATCCCC	AAGGAAAGAGCAATAGGCCG	55.0	50.0	58.0	58.0	1397-1398 bp	1.0	81.6
trnT-GGU-psbD_p2	GGTTCAGGTTACGAATCCCC	CTGCTACACCAAAACTGGGT	55.0	50.0	58.0	58.0	1807-1809 bp	1.0	81.4
trnT-GGU-psbD_p3	GGTTCAGGTTACGAATCCCC	TAAGACTCCAGCAACTCCCA	55.0	50.0	58.0	58.0	1893-1895 bp	1.0	81.3
trnT-GGU-psbD_p4	GGTTCAGGTTACGAATCCCC	GGTCGCAATTGAACAGAACG	55.0	50.0	58.0	58.0	1697-1699 bp	1.0	81.3
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	CTGCTACACCAAAACTGGGT	55.0	50.0	58.1	58.0	1495-1505 bp	1.0	80.8