Hotspot

petN-psbM

LSC · 27625-28420 · 796 bp

Back to Dipodium

Pi 0.0307

MarkerSeek score 73.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 796
variable_sites: 21
pi: 0.0307
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTGGCTTGGGCT	ACGTCAAAACAGTCAGCCAA	50.0	45.0	58.0	58.3	809-905 bp	1.0	83.5
petN-psbM_p2	GGGACATCATTCGCATGGAT	ACGTCAAAACAGTCAGCCAA	50.0	45.0	58.1	58.3	830-926 bp	1.0	83.1
petN-psbM_p3	TAGTAAGTCTGGCTTGGGCT	TACGTCAAAACAGTCAGCCA	50.0	45.0	58.0	57.4	810-906 bp	1.0	80.7
petN-psbM_p4	GGGACATCATTCGCATGGAT	TACGTCAAAACAGTCAGCCA	50.0	45.0	58.1	57.4	831-927 bp	1.0	80.3
petN-psbM_p5	TAGTATGGGGAAGGAGTGGG	ACGTCAAAACAGTCAGCCAA	55.0	45.0	57.5	58.3	752-848 bp	1.0	80.1