Hotspot

atpH-atpI

LSC · 13030-13694 · 665 bp

Back to Dipodium

Pi 0.0105

MarkerSeek score 64.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 665
variable_sites: 7
pi: 0.0105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CGCAATACCTTCTACGGCTT	AACTTTGGCTGCGGCTTATA	50.0	45.0	58.1	57.9	834-841 bp	1.0	79.2
atpH-atpI_p2	TCCGATAGAAGCAAGCCCTA	AACTTTGGCTGCGGCTTATA	50.0	45.0	57.9	57.9	783-790 bp	1.0	79.1
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	AACTTTGGCTGCGGCTTATA	45.0	45.0	57.9	57.9	762-769 bp	1.0	78.6
atpH-atpI_p4	CCGATAGAAGCAAGCCCTAC	AACTTTGGCTGCGGCTTATA	55.0	45.0	57.8	57.9	782-789 bp	1.0	78.5
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	AACTTTGGCTGCGGCTTATA	50.0	45.0	57.8	57.9	761-768 bp	1.0	78.0