Hotspot

trnS-GCU-trnG-GCC

LSC · 8149-8689 · 541 bp

Back to Dipodium

Pi 0.0355
MarkerSeek score 65.50
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
541
variable_sites
17
pi
0.0355
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA CATCGTTAGCTTGGAAGGCT 50.0 50.0 58.0 58.0 1261-1322 bp 1.0 84.8
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC CATCGTTAGCTTGGAAGGCT 50.0 50.0 58.0 58.0 1260-1321 bp 1.0 84.8
trnS-GCU-trnG-GCC_p3 CAATCCGACGCTTTAGTCCA CGTTAGCTTGGAAGGCTAGG 50.0 55.0 58.0 58.1 1258-1319 bp 1.0 84.6
trnS-GCU-trnG-GCC_p4 AATCCGACGCTTTAGTCCAC CGTTAGCTTGGAAGGCTAGG 50.0 55.0 58.0 58.1 1257-1318 bp 1.0 84.6
trnS-GCU-trnG-GCC_p5 GGGATTTGAACCCTCGGTAC CATCGTTAGCTTGGAAGGCT 55.0 50.0 58.0 58.0 1304-1365 bp 1.0 84.4