Hotspot

ndhF-ycf1

SSC · 127057-127106 · 50 bp

Back to Diplostephium

Pi 0.0843

MarkerSeek score 58.00

Primer available yes

Label ndhF-ycf1

Feature Metadata

label_name: ndhF-ycf1
feature_type: igs
region: SSC
length_bp: 50
variable_sites: 9
pi: 0.0843
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.48
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-ycf1_p1	CGTATTTCTTCTTATCTGTTCTT	TGTTTCGATGCAACAGCAAG	30.4	45.0	52.4	57.6	176-238 bp	1.0	48.9
ndhF-ycf1_p2	CGTATTTCTTCTTATCTGTTCTTT	TGTTTCGATGCAACAGCAAG	29.2	45.0	53.2	57.6	176-238 bp	1.0	48.9
ndhF-ycf1_p3	CGTATTTCTTCTTATCTGTTCTT	GTTTCGATGCAACAGCAAGA	30.4	45.0	52.4	57.3	175-237 bp	1.0	48.9
ndhF-ycf1_p4	CGTATTTCTTCTTATCTGTTCTTTT	TGTTTCGATGCAACAGCAAG	28.0	45.0	53.9	57.6	176-238 bp	1.0	48.9
ndhF-ycf1_p5	CGTATTTCTTCTTATCTGTTCTTT	GTTTCGATGCAACAGCAAGA	29.2	45.0	53.2	57.3	175-237 bp	1.0	48.9