Hotspot

rpl32-ndhF

SSC · 123786-124830 · 1045 bp

Back to Diplostephium

Pi 0.00932

MarkerSeek score 53.90

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1045
variable_sites: 53
pi: 0.00932
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CCAAATATTTTTACGAATACGCT	GCATATTGATATGTCTGTTCCA	30.4	36.4	53.9	54.1	1101-1138 bp	1.0	46.7
rpl32-ndhF_p2	TCCAAATATTTTTACGAATACGCT	GCATATTGATATGTCTGTTCCA	29.2	36.4	55.3	54.1	1102-1139 bp	1.0	46.6
rpl32-ndhF_p3	TCCAAATATTTTTACGAATACGCTT	GCATATTGATATGTCTGTTCCA	28.0	36.4	56.0	54.1	1102-1139 bp	1.0	46.6
rpl32-ndhF_p4	TTCCAAATATTTTTACGAATACGCT	GCATATTGATATGTCTGTTCCA	28.0	36.4	56.0	54.1	1103-1140 bp	1.0	46.6
rpl32-ndhF_p5	TCCAAATATTTTTACGAATACGC	GCATATTGATATGTCTGTTCCA	30.4	36.4	53.9	54.1	1102-1139 bp	1.0	46.6