Hotspot

trnL-UAG-rpl32

SSC · 122910-123620 · 711 bp

Back to Diplostephium

Pi 0.0102
MarkerSeek score 61.80
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
711
variable_sites
42
pi
0.0102
unique_haplotype_count
25
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC TGTACGACAAACTAATAAGTCCT 50.0 34.8 58.0 55.1 763-895 bp 0.962963 45.9
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA TGTACGACAAACTAATAAGTCCT 50.0 34.8 58.0 55.1 764-896 bp 0.962963 45.9
trnL-UAG-rpl32_p3 TCCTAAGAGCAGCGTGTCTA TGTACGACAAACTAATAAGTCCT 50.0 34.8 57.9 55.1 773-905 bp 0.962963 45.8
trnL-UAG-rpl32_p4 CCTAAGAGCAGCGTGTCTAC TGTACGACAAACTAATAAGTCCT 55.0 34.8 57.8 55.1 772-904 bp 0.962963 45.8
trnL-UAG-rpl32_p5 CTAAGAGCAGCGTGTCTACC TGTACGACAAACTAATAAGTCCT 55.0 34.8 57.8 55.1 771-903 bp 0.962963 45.8