Hotspot

psaC-ndhD

SSC · 119877-119988 · 112 bp

Back to Diplostephium

Pi 0.0321

MarkerSeek score 59.20

Primer available yes

Label psaC-ndhD

Feature Metadata

label_name: psaC-ndhD
feature_type: igs
region: SSC
length_bp: 112
variable_sites: 11
pi: 0.0321
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhD_p1	AAGAACGGAGGATTGTGTCG	AACCAAGGAAATTTGTTCGT	50.0	35.0	57.9	53.9	247-259 bp	1.0	51.3
psaC-ndhD_p2	CCAAGAACGGAGGATTGTGT	AACCAAGGAAATTTGTTCGT	50.0	35.0	57.8	53.9	249-261 bp	1.0	50.5
psaC-ndhD_p3	AAGAACGGAGGATTGTGTCG	TGTTAACCAAGGAAATTTGTTCGT	50.0	33.3	57.9	57.8	251-263 bp	1.0	50.4
psaC-ndhD_p4	AAGAGATGTGAATCCGCCTG	AACCAAGGAAATTTGTTCGT	50.0	35.0	57.7	53.9	222-234 bp	1.0	49.9
psaC-ndhD_p5	CCAAGAACGGAGGATTGTGT	TGTTAACCAAGGAAATTTGTTCGT	50.0	33.3	57.8	57.8	253-265 bp	1.0	49.5