Hotspot

rpl22-rps19

LSC · 83643-83722 · 80 bp

Back to Diplostephium

Pi 0.031

MarkerSeek score 69.70

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 80
variable_sites: 10
pi: 0.031
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TAACTCTTCGCGCTTTGTGA	ACGGATCGTATGGTAGGACA	45.0	50.0	57.9	57.6	239-258 bp	1.0	81.3
rpl22-rps19_p2	GAACGCCCACGAATTTGATC	ACGGATCGTATGGTAGGACA	50.0	50.0	57.8	57.6	261-280 bp	1.0	80.6
rpl22-rps19_p3	TAACTCTTCGCGCTTTGTGA	CGGATCGTATGGTAGGACAC	45.0	55.0	57.9	57.3	238-257 bp	1.0	78.5
rpl22-rps19_p4	AACGCCCACGAATTTGATCA	ACGGATCGTATGGTAGGACA	45.0	50.0	58.5	57.6	260-279 bp	1.0	78.3
rpl22-rps19_p5	ACGCCCACGAATTTGATCAA	ACGGATCGTATGGTAGGACA	45.0	50.0	58.5	57.6	259-278 bp	1.0	78.3