Hotspot

rpl16-rps3

LSC · 81375-82539 · 1165 bp

Back to Diplostephium

Pi 0.00713

MarkerSeek score 55.20

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1165
variable_sites: 36
pi: 0.00713
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	CAAGTGCCTGAAGAGCGTAT	ACAGTTCGAACAATCTATGGGG	50.0	45.5	58.0	58.1	1343-1400 bp	1.0	68.2
rpl16-rps3_p2	CAAGTGCCTGAAGAGCGTAT	CAGTTCGAACAATCTATGGGGT	50.0	45.5	58.0	58.1	1342-1399 bp	1.0	68.2
rpl16-rps3_p3	CAAGTGCCTGAAGAGCGTAT	AGTTCGAACAATCTATGGGGT	50.0	42.9	58.0	56.7	1341-1398 bp	1.0	67.0
rpl16-rps3_p4	GGGTTCAAGTGCCTGAAGAG	ACAGTTCGAACAATCTATGGGG	55.0	45.5	58.5	58.1	1348-1405 bp	1.0	64.6
rpl16-rps3_p5	GGGTTCAAGTGCCTGAAGAG	CAGTTCGAACAATCTATGGGGT	55.0	45.5	58.5	58.1	1347-1404 bp	1.0	64.6