Hotspot

psbZ-trnG-UCC

LSC · 35878-36189 · 312 bp

Back to Diplostephium

Pi 0.00948

MarkerSeek score 41.30

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 312
variable_sites: 12
pi: 0.00948
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	CAACCACTCAGCCATCTCTC	GGGTAGAGCAGTTTGGTAGC	55.0	55.0	58.0	58.0	1185-1261 bp	1.0	84.6
psbZ-trnG-UCC_p2	GGTTTTCAAGACCGGGACTT	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.0	58.0	1206-1282 bp	1.0	84.5
psbZ-trnG-UCC_p3	GACTTTCAACCACTCAGCCA	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.0	58.0	1191-1267 bp	1.0	84.5
psbZ-trnG-UCC_p4	TCCATTTTTCTGGCTTGGCT	GGGTAGAGCAGTTTGGTAGC	45.0	55.0	58.0	58.0	1361-1437 bp	1.0	84.1
psbZ-trnG-UCC_p5	TCCATTTTTCTGGCTTGGCT	CTTGAGGTCACGGGTTCAAA	45.0	50.0	58.0	58.0	1325-1401 bp	1.0	84.1