Hotspot

trnG-UCC-trnT-GGU

LSC · 30806-31223 · 418 bp

Back to Diplostephium

Pi 0.0341
MarkerSeek score 70.30
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
418
variable_sites
78
pi
0.0341
unique_haplotype_count
26
primer_available
yes
alignment_reliability
0.83
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 AATGAAACCGAAAGACCCCT TCCCCTATCGGATCTGAACC 45.0 55.0 57.0 58.0 368-561 bp 0.888889 79.7
trnG-UCC-trnT-GGU_p2 ATGAAACCGAAAGACCCCTT TCCCCTATCGGATCTGAACC 45.0 55.0 57.0 58.0 367-560 bp 0.888889 79.7
trnG-UCC-trnT-GGU_p3 GAATGAAACCGAAAGACCCCT TCCCCTATCGGATCTGAACC 47.6 55.0 58.2 58.0 369-562 bp 0.888889 77.8
trnG-UCC-trnT-GGU_p4 AATGAAACCGAAAGACCCCT GATCTGAACCGAGGGCTTAC 45.0 55.0 57.0 57.8 358-551 bp 0.851852 76.5
trnG-UCC-trnT-GGU_p5 ATGAAACCGAAAGACCCCTT GATCTGAACCGAGGGCTTAC 45.0 55.0 57.0 57.8 357-550 bp 0.851852 76.5