Hotspot

trnI-CAU-rrn16

Genome · 99931-105448 · 5518 bp

Back to Diplopterygium

Pi 0.014
MarkerSeek score 52.50
Primer available yes
Label trnI-CAU-rrn16

Feature Metadata

label_name
trnI-CAU-rrn16
feature_type
igs
region
Genome
length_bp
5518
variable_sites
77
pi
0.014
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-CAU-rrn16_p1 GTTAGGCGGGATGTTCTGAG GCTGCTACACGTCAATACCA 55.0 50.0 58.1 58.0 1201-1318 bp 1.0 85.4
trnI-CAU-rrn16_p2 GTTAGGCGGGATGTTCTGAG TGCTGCTACACGTCAATACC 55.0 50.0 58.1 58.0 1202-1319 bp 1.0 85.4
trnI-CAU-rrn16_p3 ATTGAACTAGGGCTGGGACT GCTGCTACACGTCAATACCA 50.0 50.0 58.0 58.0 1411-1555 bp 1.0 84.8
trnI-CAU-rrn16_p4 ATTGAACTAGGGCTGGGACT TGCTGCTACACGTCAATACC 50.0 50.0 58.0 58.0 1412-1556 bp 1.0 84.8
trnI-CAU-rrn16_p5 TTAGGCGGGATGTTCTGAGA GCTGCTACACGTCAATACCA 50.0 50.0 58.1 58.0 1200-1317 bp 1.0 84.8