Hotspot

rps8-rpl14

Genome · 94083-94518 · 436 bp

Back to Diplopterygium

Pi 0.0257

MarkerSeek score 65.50

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: Genome
length_bp: 436
variable_sites: 11
pi: 0.0257
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	CGGGTATTCGAACCGTAGTC	TCTTCGGTCCAGTAGCTAGG	55.0	55.0	57.9	57.9	587-595 bp	1.0	81.2
rps8-rpl14_p2	CCCGTACTGGCATTTCGTAT	TCTTCGGTCCAGTAGCTAGG	50.0	55.0	57.8	57.9	561-569 bp	1.0	80.4
rps8-rpl14_p3	CGGGTATTCGAACCGTAGTC	CCAGAGTCTTCGGTCCAGTA	55.0	55.0	57.9	58.2	593-601 bp	1.0	80.3
rps8-rpl14_p4	GTCTTTCTTCCCGTACTGGC	TCTTCGGTCCAGTAGCTAGG	55.0	55.0	58.3	57.9	570-578 bp	1.0	80.0
rps8-rpl14_p5	CGGGTATTCGAACCGTAGTC	TGTGGTGAATCAGGAGGGAA	55.0	50.0	57.9	58.3	625-633 bp	1.0	79.5