Hotspot

rpl20-rps12

Genome · 82044-82959 · 916 bp

Back to Diplopterygium

Pi 0.00437

MarkerSeek score 61.30

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: Genome
length_bp: 916
variable_sites: 4
pi: 0.00437
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TGTTGGTTCGCTGTTCTGAA	TGGACATGCCAACCAATCAA	45.0	45.0	58.0	58.0	1148 bp	1.0	80.0
rpl20-rps12_p2	TTGTTGGTTCGCTGTTCTGA	TGGACATGCCAACCAATCAA	45.0	45.0	58.0	58.0	1149 bp	1.0	80.0
rpl20-rps12_p3	CGCTGTTCTGAACGATTTCG	TGGACATGCCAACCAATCAA	50.0	45.0	57.9	58.0	1140 bp	1.0	79.2
rpl20-rps12_p4	TGTTGGTTCGCTGTTCTGAA	AGGCAAGACAACCGATGAAG	45.0	50.0	58.0	58.2	1115 bp	1.0	78.5
rpl20-rps12_p5	TTGTTGGTTCGCTGTTCTGA	AGGCAAGACAACCGATGAAG	45.0	50.0	58.0	58.2	1116 bp	1.0	78.5