Hotspot

rps4-trnL-UAA

Genome · 60748-61705 · 958 bp

Back to Diplopterygium

Pi 0.0353

MarkerSeek score 76.00

Primer available yes

Label rps4-trnL-UAA

Feature Metadata

label_name: rps4-trnL-UAA
feature_type: igs
region: Genome
length_bp: 958
variable_sites: 33
pi: 0.0353
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL-UAA_p1	AGCCCTGGTAAATCTTTCAGAC	GAGCCATCACTCGATGTGTT	45.5	50.0	58.0	58.0	1084-1103 bp	1.0	67.7
rps4-trnL-UAA_p2	TGTTTTGTTAGTAAGCCCTGGT	GAGCCATCACTCGATGTGTT	40.9	50.0	57.8	58.0	1097-1116 bp	1.0	66.6
rps4-trnL-UAA_p3	AGCCCTGGTAAATCTTTCAGAC	CCTGATTTTGGGAGCCATCA	45.5	50.0	58.0	57.9	1095-1114 bp	1.0	66.6
rps4-trnL-UAA_p4	CCCTGGTAAATCTTTCAGACGA	GAGCCATCACTCGATGTGTT	45.5	50.0	57.8	58.0	1082-1101 bp	1.0	66.4
rps4-trnL-UAA_p5	TGGTAAATCTTTCAGACGACGT	GAGCCATCACTCGATGTGTT	40.9	50.0	57.8	58.0	1079-1098 bp	1.0	66.1