Hotspot

trnS-UGA-psbC

Genome · 46669-47486 · 818 bp

Back to Diplopterygium

Pi 0.0037

MarkerSeek score 61.10

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: Genome
length_bp: 818
variable_sites: 3
pi: 0.0037
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	ATGGTCGAGTGGTTTATGGC	CCTATGGCATGCAGGAAGAG	50.0	55.0	58.0	58.1	996-1001 bp	1.0	79.6
trnS-UGA-psbC_p2	TTCGAATCCCTCTCTCTCCC	CCTATGGCATGCAGGAAGAG	55.0	55.0	57.9	58.1	933-938 bp	1.0	79.4
trnS-UGA-psbC_p3	AGTGCTGAAACGCTATCGAG	CCTATGGCATGCAGGAAGAG	50.0	55.0	58.1	58.1	955-960 bp	1.0	79.3
trnS-UGA-psbC_p4	ATGGTCGAGTGGTTTATGGC	CTTCTTCTTCGTGGGTCACC	50.0	55.0	58.0	58.2	1014-1019 bp	1.0	78.9
trnS-UGA-psbC_p5	ATGGTCGAGTGGTTTATGGC	AGCCGGGTTTGAAAAAGGAA	50.0	45.0	58.0	58.2	963-968 bp	1.0	78.8