Hotspot

psbM-trnD-GUC

Genome · 44088-44749 · 662 bp

Back to Diplopterygium

Pi 0.0121

MarkerSeek score 65.90

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: Genome
length_bp: 662
variable_sites: 8
pi: 0.0121
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TGGAGAAGCTGTGGGGATTA	TAGAAGTGCTTCAACGAGCC	50.0	50.0	58.0	57.9	825-826 bp	1.0	80.0
psbM-trnD-GUC_p2	ATTGTTTTGAGCGACCGTCT	TAGAAGTGCTTCAACGAGCC	45.0	50.0	58.1	57.9	861-862 bp	1.0	79.2
psbM-trnD-GUC_p3	TTTTGAGCGACCGTCTGAAT	TAGAAGTGCTTCAACGAGCC	45.0	50.0	57.8	57.9	857-858 bp	1.0	78.9
psbM-trnD-GUC_p4	GAGCGACCGTCTGAATGTAA	TAGAAGTGCTTCAACGAGCC	50.0	50.0	57.7	57.9	853-854 bp	1.0	78.0
psbM-trnD-GUC_p5	TTTGAGCGACCGTCTGAATG	TAGAAGTGCTTCAACGAGCC	50.0	50.0	58.6	57.9	856-857 bp	1.0	75.6