Hotspot

rps16-chlB

Genome · 19281-19461 · 181 bp

Back to Diplopterygium

Pi 0.0276

MarkerSeek score 65.80

Primer available yes

Label rps16-chlB

Feature Metadata

label_name: rps16-chlB
feature_type: igs
region: Genome
length_bp: 181
variable_sites: 5
pi: 0.0276
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-chlB_p1	GATTTGGGGATGGCGGTTAT	CCGGTTTTGTGAGGGGAAAA	50.0	50.0	58.0	58.6	395-413 bp	1.0	76.2
rps16-chlB_p2	ACGTCGCTTTCCACCATATC	CCGGTTTTGTGAGGGGAAAA	50.0	50.0	58.1	58.6	339-357 bp	1.0	75.9
rps16-chlB_p3	GTCGCTTTCCACCATATCGT	CCGGTTTTGTGAGGGGAAAA	50.0	50.0	58.1	58.6	337-355 bp	1.0	75.9
rps16-chlB_p4	TCTCGATGTTTGAGCCACAC	CCGGTTTTGTGAGGGGAAAA	50.0	50.0	58.2	58.6	357-375 bp	1.0	74.7
rps16-chlB_p5	ACTCCGCAAGAATGATCTCG	CCGGTTTTGTGAGGGGAAAA	50.0	50.0	57.8	58.6	372-390 bp	1.0	74.6