Hotspot

matK-rps16

Genome · 17044-18160 · 1117 bp

Back to Diplopterygium

Pi 0.00627

MarkerSeek score 62.40

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: Genome
length_bp: 1117
variable_sites: 7
pi: 0.00627
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	AGCATTCCCATTTTGGATCGA	CTGCCATCATTTCCTTCCCA	42.9	50.0	58.0	57.9	1303 bp	1.0	71.1
matK-rps16_p2	AGCATTCCCATTTTGGATCGA	TGCCATCATTTCCTTCCCAG	42.9	50.0	58.0	57.9	1302 bp	1.0	71.1
matK-rps16_p3	AGCATTCCCATTTTGGATCGA	GGGCTCAAACCACAGAAACT	42.9	50.0	58.0	58.3	1276 bp	1.0	69.8
matK-rps16_p4	AGCATTCCCATTTTGGATCGA	GCCATCATTTCCTTCCCAGA	42.9	50.0	58.0	57.6	1301 bp	1.0	68.8
matK-rps16_p5	AGCATTCCCATTTTGGATCGA	TCTGCCATCATTTCCTTCCC	42.9	50.0	58.0	57.6	1304 bp	1.0	68.8