Hotspot

ccsA

SSC · 115716-116669 · 954 bp

Back to Diplolaena

Pi 0.0126

MarkerSeek score 55.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 954
variable_sites: 12
pi: 0.0126
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	ACAAAAGGCGCTCTAAGGAA	ATGCGCTGTCCTATGTTTGT	45.0	45.0	57.4	57.9	1085 bp	1.0	75.5
ccsA_p2	ACAAAAGGCGCTCTAAGGAA	GCGCTGTCCTATGTTTGTCT	45.0	50.0	57.4	58.3	1083 bp	1.0	74.2
ccsA_p3	ACAAAAGGCGCTCTAAGGAA	TGCGCTGTCCTATGTTTGTC	45.0	50.0	57.4	58.6	1084 bp	1.0	71.9
ccsA_p4	ACAAAAGGCGCTCTAAGGAAT	ATGCGCTGTCCTATGTTTGT	42.9	45.0	57.9	57.9	1085 bp	1.0	71.4
ccsA_p5	ACAAAAGGCGCTCTAAGGAAT	GCGCTGTCCTATGTTTGTCT	42.9	50.0	57.9	58.3	1083 bp	1.0	70.1